spicifera (73.9 %), and Alternaria alternata (73.7 %) at the concentration of 32 g L-1. amboinicus displayed the greatest inhibition on the mycelial growth of Sclerotinia sclerotiorum (83.0 %), Cochliobolus cynodontis (82.1 %), Botrytis cinerea(77.8 %), Bipolaris hawaiensis (74.1 %), B. splendens showed a remarkable selectivity toward MCF7 cell line with IC50 valueby 0.040 g L-1.The methanol extract of P. amboinicus showed the highest antitumor activity using A549, MCF7, and HEPG cell lines with IC50 values by 0.041, 0.047 and 0.043 g L-1, respectively. amboinicus recorded the lowest IC50 and EC50 values for DPPH and reducing power by 0.042 g L-1, and 0.327 g L-1, respectively, indicating the greatest antioxidant potential. Carvacrol was the predominant component (7.59%) of P.amboinicus extract by GC-MS analysis. Rosmarinic acid (1.021 g Kg-1) was the predominant phenol in P. amboinicus extract containsthe highest contents of the total polyphenols and total flavonoids by 122.2 g GAE Kg-1 and 41.4 g QE Kg-1, respectively. The present study aims to elucidate the chemical characterization of the methanolic extracts of Coleus blumei, Plectranthus amboinicus, and Salvia splendens with emphasis on their antioxidant, antifungal, and cytotoxic activities. This research shows that methanolic extract of Miana leaves can be used as an alternative to botanical pesticides in controlling houseflies (Musca domestica). The results of the study revealed that at a concentration of 30% and 40%, methanolic extract of Miana leaves could result in a knockdown effect that was accompanied by death with a percentage value of 100% in the first 5 minutes of observation. The results of phytochemical screening with chemical reagents showed that the extract contained alkaloids, tannins, flavonoids, saponins and terpenoids while the results of GCMS analysis showed 8 bioactive compounds.
Testing of methanolic extract of Miana leaves was made in the form of spray using the knockdown method at concentrations of 5%, 10%, 20%, 30% and 40% and observed in 5, 10 and 15 minutes. Miana leaf fine powder was soaked with methanol (maceration) for 3 days followed byphytochemical screening (chemical reagents and Gas Chromatography Mass Spectrophotometry (GCMS) and anti-houseflies toxicity tests. The purpose of this study was to determine the chemical compounds contained in Miana leaf as well as determine the optimal concentration of Miana leaf extract in causing knockdown effect in houseflies. One way to control houseflies is using Miana leaves (Coleus blumei) as botanical pesticide. If you’re looking for a rapidly growing business venture that can be started at a low cost and be run with very small overhead, then Vape & Smoke shop may be the the perfect fit for you.Houseflies (Musca domestica) is an disease-carrying insect that plays an important role in the spread of disease in humans and animals.
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